Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5556] to Pannexin 1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free
    See all Pannexin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5556] to Pannexin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248078 is the carrier-free version of ab124969. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248078 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    Western blot - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)

    Lane 1 : Caco-2 whole cell lysate
    Lane 2 : NIH:OVCAR-3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    This data was developed using ab124969, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    Immunoprecipitation - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    This data was developed using ab124969, the same antibody clone in a different buffer formulation.ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate. Lane 1 (input): SH-SY5Y whole cell lysate (10µg) Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate. Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration: 5% NFDM /TBST.
  • Western blot - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    Western blot - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal brain cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : Caco2 cell lysates
    Lane 4 : NIH OVCAR-3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 48 kDa



    This data was developed using ab124969, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    OI-RD Scanning - Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    This data was developed using ab124969, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)
    Anti-Pannexin 1 antibody [EPR5556] - BSA and Azide free (ab248078)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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