Anti-Neurofibromin antibody (ab17963)
Key features and details
- Rabbit polyclonal to Neurofibromin
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Neurofibromin antibody
See all Neurofibromin primary antibodies -
Description
Rabbit polyclonal to Neurofibromin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T, TCMK-1 and NIH3T3 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Neurofibromin antibody (ab17963)All lanes : Anti-Neurofibromin antibody (ab17963) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate in NETN lysis buffer
Lane 2 : 293T whole cell lysate in NETN lysis buffer
Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer
Lane 5 : NIH3T3 whole cell lysate in NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 319 kDa
Exposure time: 75 seconds
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Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)Whole cell lysate (0.5-1.0mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: IP using rabbit anti-Neurofibromin antibody.
Lane 2: IP using ab17963 at 6 µg per reaction.
Lane 3: Control IgG.For western blotting, ab17963 was used at 1 µg/ml.
