Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2573(2)] to NF2 / Merlin - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free
    See all NF2 / Merlin primary antibodies

  • Description

    Rabbit monoclonal [EPR2573(2)] to NF2 / Merlin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa whole cell lysate (ab150035), MCF7 cell lysate, Jurkat whole cell lysate (ab7899), PC3 cell lysate

  • General notes

    Ab247805 is the carrier-free version of ab109244. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247805 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    All lanes : Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NF2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab109244).

    Lanes 1 - 2: Merged signal (red and green). Green - ab109244 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab109244 was shown to react with NF2 in wild-type HeLa cells in western blot with loss of signal observed in NF2 knockout cell line ab261796 (NF2 knockout cell lysate ab257179). Wild-type and NF2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab109244 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)

    This data was developed using ab109244, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)

    Lane 2: NF2 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab109244 observed at 70 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab109244 was shown to specifically react with NF2 in wild type cells as signal was lost in NF2 knockout cells. Wild-type and NF2 knockout samples were subjected to SDS-PAGE. ab109244 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    All lanes : Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 70 kDa



    This data was developed using ab109244, the same antibody clone in a different buffer formulation.

     

  • Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)
    Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (ab247805)

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