Human JAK2 knockout A549 cell pellet (ab278814)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human JAK2 knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human JAK2 knockout A549 cell pellet (ab278814)Lane 1: Wild-type A549 cell lysate (20µg)
Lane 2: JAK2 knockout A549 cell lysate (20µg)
Lane 3: K562 cell lysate (20µg)
Lane 4: Daudi cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab108596 observed at 131 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab108596 Anti-JAK2 antibody [EPR108(2)] was shown to specifically react with JAK2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267113 (knockout cell lysate ab256963) was used. Wild-type and JAK2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108596 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human JAK2 knockout A549 cell pellet (ab278814)Allele-1: 5 bp deletion in exon3
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Sanger Sequencing - Human JAK2 knockout A549 cell pellet (ab278814)Allele-2: 1 bp insertion in exon3