Anti-Pannexin 1 antibody [EPR5556] (ab124969)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5556] to Pannexin 1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Pannexin 1 antibody [EPR5556]
See all Pannexin 1 primary antibodies -
Description
Rabbit monoclonal [EPR5556] to Pannexin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB: Human fetal brain tissue lysate and SH-SY5Y, Caco2, and NIH OVCAR-3 cell lysates. IP: SH-SY5Y cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.52 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5556 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)
Lane 1 : Caco-2 whole cell lysate
Lane 2 : NIH:OVCAR-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y whole cell lysate (10µg)
Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : Caco2 cell lysates
Lane 4 : NIH OVCAR-3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 48 kDa
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