Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432) - BSA and Azide free
  • Suitable for: Dot blot, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free
    See all p95/NBS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects p95/NBS1 that is phosphorylated on serine 432.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247400 is the carrier-free version of ab75778 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247400 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as p95 NBS1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    Dot Blot - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    This data was developed using ab75778, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: p95/NBS1 (pS432) phospho peptide Lane 2 sample: p95/NBS1 non-phospho peptide Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    This data was developed using ab75778, the same antibody clone in a different buffer formulation.ab75778 staining p95/NBS1 (phospho S432) in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    This data was developed using ab75778, the same antibody clone in a different buffer formulation.ab75778 at 1/100-1/250 dilution staining p95/NBS1 in human testis by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)
    Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] - BSA and Azide free (ab247400)

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