Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNF7 antibody [EPR12001] (ab181986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12001] to RNF7
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RNF7 antibody [EPR12001]
    See all RNF7 primary antibodies

  • Description

    Rabbit monoclonal [EPR12001] to RNF7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder  tissue labeling RNF7 with
    ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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