Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)
![Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-445631-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-western-blot.jpg "Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17353] to p53 (phospho S37) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
-
Product name
Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR17353] to p53 (phospho S37) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours; HEK-293 and A431 whole cell lysates.
-
General notes
Ab250606 is the carrier-free version of ab182164. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250606 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17353 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : A431 treated with 1μg/ml doxorubicin for 24 hours whole cell lysate
Lane 3 : A431 treated with 1μg/ml doxorubicin for 24 hours whole cell lysate, the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab182164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-410352-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/50000 dilution
Lane 1 : p53 transfected HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab182164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-407916-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution
Lane 1 : A431 whole cell lysate (Human epidermoid carcinoma)
Lane 2 : A431 whole cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab182164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-3-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)This data was developed using ab182164, the same antibody clone in a different buffer formulation.Dot blot analysis of p53 (phospho S37) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab182164 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time: 30 seconds. -
![Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-403502-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution
Lane 1 : HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : HEK-293 whole cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab182164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-398022-anti-p53-phospho-s37-antibody-epr17353-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution + Saos-2 whole cell lysate (Human osteosarcoma cell line) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Exposure time: 3 minutesThis data was developed using ab182164, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Saos-2 doesn't express p53.
-
![Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)](https://www.abcam.com/ps/products/250/ab250606/Images/ab250606-7-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S37) antibody [EPR17353] - BSA and Azide free (ab250606)