Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
![Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)](https://www.abcam.com/ps/products/275/ab275029/Images/ab275029-2-anti-b7h6-antibody-epr23470210-western-blot-liver.jpg "Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23470-210] to B7-H6 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free
See all B7-H6 primary antibodies -
Description
Rabbit monoclonal [EPR23470-210] to B7-H6 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and K562 whole cell lysates. IP: HepG2 whole cell lysate.
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General notes
ab275029 is the carrier-free version of ab253180. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23470-210 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)All lanes : Anti-B7-H6 antibody [EPR23470-210] (ab253180) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate treated with PNGase F 20
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?This data was developed using ab253180, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: MCF7.
The expression profile is consistent with the literature (PMID: 22851709).
B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID: 24780758). -
![Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)](https://www.abcam.com/ps/products/275/ab275029/Images/ab275029-1-anti-b7h6-antibody-epr23470210-immunoprecipitation-hepg2.jpg)
Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)This data was developed using ab253180, the same antibody clone in a different buffer formulation.
B7-H6 was immunoprecipitated from 0.35 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab253180 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate 10 μg
Lane 2: ab253180 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253180 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
The lower bands are likely to be the deglycosylated form of B7-H6
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![Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)](https://www.abcam.com/ps/products/275/ab275029/Images/ab275029-3-benefits-of-recombinant-antibodies.png)
Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
