Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23470-210] to B7-H6 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free
    See all B7-H6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23470-210] to B7-H6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and K562 whole cell lysates. IP: HepG2 whole cell lysate.

  • General notes

    ab275029 is the carrier-free version of ab253180. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23470-210

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
    Western blot - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
    All lanes : Anti-B7-H6 antibody [EPR23470-210] (ab253180) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 whole cell lysate treated with PNGase F 20
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 50,80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab253180, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: MCF7.

    The expression profile is consistent with the literature (PMID: 22851709).
    B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID: 24780758).

  • Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
    Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)

    This data was developed using ab253180, the same antibody clone in a different buffer formulation.

    B7-H6 was immunoprecipitated from 0.35 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate  with ab253180 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate 10 μg

    Lane 2: ab253180 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253180 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds.

    The lower bands are likely to be the deglycosylated form of B7-H6

  • Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)
    Anti-B7-H6 antibody [EPR23470-210] - BSA and Azide free (ab275029)

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