Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPFHCR16] to p27 KIP 1 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat

Product name

Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free
See all p27 KIP 1 primary antibodies
Description

Rabbit monoclonal [EPFHCR16] to p27 KIP 1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment within Mouse p27 KIP 1. The exact sequence is proprietary.
General notes
ab247597 is the carrier-free version of ab92741 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247597 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPFHCR16
Isotype

IgG
Research areas

Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

All lanes : Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

Lane 1 : NIH/3T3 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Neuro-2a cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 22 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

This data was developed using ab92741, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

This data was developed using ab92741, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

This data was developed using ab92741, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

This data was developed using ab92741, the same antibody clone in a different buffer formulation.ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

This data was developed using ab92741, the same antibody clone in a different buffer formulation.ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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