Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPFHCR16] to p27 KIP 1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free
    See all p27 KIP 1 primary antibodies

  • Description

    Rabbit monoclonal [EPFHCR16] to p27 KIP 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse p27 KIP 1. The exact sequence is proprietary.

  • General notes

    ab247597 is the carrier-free version of ab92741 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247597 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    All lanes : Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92741, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    This data was developed using ab92741, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    This data was developed using ab92741, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    This data was developed using ab92741, the same antibody clone in a different buffer formulation.ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    This data was developed using ab92741, the same antibody clone in a different buffer formulation.ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)
    Anti-p27 KIP 1 antibody [EPFHCR16] - BSA and Azide free (ab247597)

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