Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Orexin B antibody [EPR22803-18] (ab255293)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22803-18] to Orexin B
Suitable for: Dot blot, IHC-Fr, IHC-P, ELISA
Reacts with: Mouse, Rat

Product name

Anti-Orexin B antibody [EPR22803-18]
See all Orexin B primary antibodies
Description

Rabbit monoclonal [EPR22803-18] to Orexin B
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Mouse
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Dot Blot: Orexin B peptide (aa69-96). IHC-P: Mouse and rat hypothalamus tissue. IHC-Fr: Mouse and rat hypothalamus tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22803-18
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Orexin B with ab255293 at 1/1000 dilution (0.468 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hypothalamus (PMID: 9419374) is observed. The section was incubated with ab255293 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling Orexin B with ab255293 at 1/500 (0.94 μg/ml) dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Positive staining on hypothalamic neurons (PMID: 9822755).
ELISA - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Direct ELISA antigen dose-response curve using ab255293 at 1 µg/mL. Antigen (mouse Orexin B peptide) concentration of 0.488-500 ng/mL. ab97048 Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) at 1/5000 was used as the secondary antibody.
Dot Blot - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Dot Blot - Anti-Orexin B antibody [EPR22803-18] (ab255293 at a 1/1000 dilution).

Lane 1: Orexin A peptide (aa33-65).

Lane 2: Orexin B peptide (aa69-96).

Blocking/Dilution buffer: 5% NFDM/TBST.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 at a 1/100000 dilution) secondary antibody.

Exposure time 3 mins.
Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling Orexin B with ab255293 at 1/500 (0.94 μg/ml) dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Positive staining on hypothalamic neurons (PMID: 9822755).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] (ab255293)

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Orexin B with ab255293 at 1/1000 dilution (0.468 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat hypothalamus (PMID: 9419374) is observed. The section was incubated with ab255293 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-Orexin B antibody [EPR22803-18] (ab255293)

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