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Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22803-18] to Orexin B - BSA and Azide free
  • Suitable for: Dot blot, IHC-Fr, IHC-P, ELISA
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free
    See all Orexin B primary antibodies

  • Description

    Rabbit monoclonal [EPR22803-18] to Orexin B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IHC-Fr, IHC-P, ELISAmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot Blot: Orexin B peptide (aa69-96). IHC-P: Mouse and rat hypothalamus tissue. IHC-Fr: Mouse and rat hypothalamus tissue.

  • General notes

    ab256513 is the carrier-free version of ab255293.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Orexin B with ab255293 at 1/1000 dilution (0.468 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hypothalamus (PMID: 9419374) is observed. The section was incubated with ab255293 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling Orexin B with ab255293 at 1/500 (0.94 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    Positive staining on hypothalamic neurons (PMID: 9822755).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • ELISA - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    ELISA - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Direct ELISA antigen dose-response curve using ab255293 at 1 µg/mL. Antigen (mouse Orexin B peptide) concentration of 0.488-500 ng/mL. ab97048 Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) at 1/5000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • Dot Blot - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Dot Blot - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Dot Blot - Anti-Orexin B antibody [EPR22803-18] (ab255293 at a 1/1000 dilution).

     

    Lane 1: Orexin A peptide (aa33-65).

    Lane 2: Orexin B peptide (aa69-96).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 at a 1/100000 dilution) secondary antibody.

    Exposure time 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Immunohistochemistry (Frozen sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling Orexin B with ab255293 at 1/500 (0.94 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    Positive staining on hypothalamic neurons (PMID: 9822755).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus  tissue labeling Orexin B with ab255293 at 1/1000 dilution (0.468 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat hypothalamus (PMID: 9419374) is observed. The section was incubated with ab255293 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255293).

  • Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)
    Anti-Orexin B antibody [EPR22803-18] - BSA and Azide free (ab256513)

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