Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)
![Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-445620-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-western-blot.jpg "Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH - BSA and Azide free
- Suitable for: ICC, IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free
See all ALDH4A1/P5CDH primary antibodies -
Description
Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250469 is the carrier-free version of ab181256 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ALDH4A1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14288(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)All lanes : Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDaThis data was developed using ab181256, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-410323-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDaThis data was developed using ab181256, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-4-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)This data was developed using ab181256, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-3-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)This data was developed using ab181256, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-2-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)This data was developed using ab181256, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling ALDH4A1/P5CDH with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining. -
![Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-399625-anti-aldh4a1-p5cdh-antibody-epr14288-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)This data was developed using ab181256, the same antibody clone in a different buffer formulation.
Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1/P5CDH, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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![Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)](https://www.abcam.com/ps/products/250/ab250469/Images/ab250469-6-benefits-of-recombinant-antibodies.png)
Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free (ab250469)