Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

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Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5662(2)] to Nuclear Matrix Protein p84
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)]
See all Nuclear Matrix Protein p84 primary antibodies
Description

Rabbit monoclonal [EPR5662(2)] to Nuclear Matrix Protein p84
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nuclear Matrix Protein p84 aa 350-450. The exact sequence is proprietary.
Positive control
- WB: HeLa, U-87 MG, 293T and HepG2 (ab7900) cell lysates. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5662(2)
Isotype

IgG
Research areas

Western blot - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 76 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

Immunofluorescent staining of HeLa cells labelling Nuclear Matrix Protein p84 with ab131268 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labeling Nuclear Matrix Protein p84 with ab131268 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Nuclear Matrix Protein p84 with purified ab131268 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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