Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)
![Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)](https://www.abcam.com/ps/products/125/ab125019/Images/ab125019-139145-anti-nuclear-matrix-protein-p84-antibody-epr5661-western-blot.jpg "Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5661] to Nuclear Matrix Protein p84
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Nuclear Matrix Protein p84 antibody [EPR5661]
See all Nuclear Matrix Protein p84 primary antibodies -
Description
Rabbit monoclonal [EPR5661] to Nuclear Matrix Protein p84 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Nuclear Matrix Protein p84 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, U-87 MG and 293T cell lysates; Human stomach adenocarcinoma tissue; 293T cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5661 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)](https://www.abcam.com/ps/products/125/ab125019/Images/ab125019-139144-anti-nuclear-matrix-protein-p84-antibody-epr5661-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)ab125019, at 1/50 dilution, staining Nuclear Matrix Protein p84 in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)](https://www.abcam.com/ps/products/125/ab125019/Images/ab125019-139143-anti-nuclear-matrix-protein-p84-antibody-epr5661-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)ab125019, at 1/100 dilution, staining Nuclear Matrix Protein p84 in 293T cells by Immunofluorescence. -
![Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)](https://www.abcam.com/ps/products/125/ab125019/Images/ab125019-4-benefits-of-recombinant-antibodies.png)
Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)
