Anti-NLE1/nle antibody (ab254731)
Key features and details
- Rabbit polyclonal to NLE1/nle
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NLE1/nle antibody
See all NLE1/nle primary antibodies -
Description
Rabbit polyclonal to NLE1/nle -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human NLE1/nle aa 250-400.
Database link: Q9NVX2 -
Positive control
- IHC-P: Human oral mucosa tissue. WB: RT4, NIH/3T3, NBT-II and U-251 MG cell lysate. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLE1/nle antibody (ab254731)Paraffin-embedded human oral mucosa tissue stained for NLE1/nle using ab254731 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-NLE1/nle antibody (ab254731)Lanes 2-3 : Anti-NLE1/nle antibody (ab254731) at 0.4 µg/ml
Lane 1 : MW Ladder
Lane 2 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 3 : U-251 MG (Human brain glioma cell line) cell lysate
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Western blot - Anti-NLE1/nle antibody (ab254731)Lanes 2-3 : Anti-NLE1/nle antibody (ab254731) at 0.4 µg/ml
Lane 1 : MW Ladder
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 3 : NBT-II (Rat bladder carcinoma cell line) cell lysate
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Immunocytochemistry/ Immunofluorescence - Anti-NLE1/nle antibody (ab254731)PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling NLE1/nle using ab254731 at 4µg/ml (green) in ICC/IF.
