Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPS1 antibody [EPR20843] (ab216869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20843] to DPS1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DPS1 antibody [EPR20843]
    See all DPS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20843] to DPS1

  • Host species

    Rabbit

  • Specificity

    collaborative project

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HeLa, A549, K-562, Jurkat, F9, C6, and RAW 264.7 cell lysate. IP: MCF7 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
    Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
    All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 3 minutes.

    The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

  • Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
    Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 3 : RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time:
    Lane 1: 3 minutes
    Lane 2-3: 15 seconds.

    The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

  • Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)
    Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)

    DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
    Lane 2: ab216869 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 150 seconds.

  • Anti-DPS1 antibody [EPR20843] (ab216869)
    Anti-DPS1 antibody [EPR20843] (ab216869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DPS1 antibody [EPR20843] (ab216869)

© 2021 Astana Biomed Group LLP. All rights reserved.