Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-DPS1 antibody [EPR20843] (ab216869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20843] to DPS1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-DPS1 antibody [EPR20843]
See all DPS1 primary antibodies
Description

Rabbit monoclonal [EPR20843] to DPS1
Host species

Rabbit
Specificity

collaborative project
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, HeLa, A549, K-562, Jurkat, F9, C6, and RAW 264.7 cell lysate. IP: MCF7 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20843
Isotype

IgG
Research areas

Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)

All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 minutes.

The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.
Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)

All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 3 : RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure time:
Lane 1: 3 minutes
Lane 2-3: 15 seconds.

The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.
Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)

DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
Lane 2: ab216869 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 150 seconds.
Anti-DPS1 antibody [EPR20843] (ab216869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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