Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20843] to DPS1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DPS1 antibody [EPR20843] - BSA and Azide free
    See all DPS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20843] to DPS1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    collaborative project

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HeLa, A549, K-562, Jurkat and F9 cell lysate.

  • General notes

    Ab233697 is the carrier-free version of ab216869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab233697 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)
    Immunoprecipitation - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)

    DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
    Lane 2: ab216869 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 150 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

  • Western blot - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)
    Western blot - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)
    All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 3 minutes.

    The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

  • Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)
    Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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