Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16230-47] to NDUFB10 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free
    See all NDUFB10 primary antibodies

  • Description

    Rabbit monoclonal [EPR16230-47] to NDUFB10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251216 is the carrier-free version of ab196019. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251216 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Western blot - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    All lanes : Anti-NDUFB10 antibody [EPR16230-47] (ab196019) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab196019, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    This data was developed using ab196019, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NDUFB10 with ab196019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin. Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Immunocytochemistry - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    This data was developed using ab196019, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NDUFB10 with ab196019 at 1/350 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue).
  • Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    This data was developed using ab196019, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB10 (red) with purified ab196019 at a dilution of 1/800. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.
  • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    This data was developed using ab196019, the same antibody clone in a different buffer formulation.NDUFB10 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196019 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196019 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196019 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
    Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)

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