Anti-NCAM1 antibody (ab118291)
Key features and details
- Goat polyclonal to NCAM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCAM1 antibody
See all NCAM1 primary antibodies -
Description
Goat polyclonal to NCAM1 -
Host species
Goat -
Specificity
ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
KASWTRPEKQETLD
with a Cysteine residue linker, corresponding to internal sequence amino acids 353-366 of Human NCAM (NP_851996.2). -
Positive control
- WB: Human brain frontal cortex lysates; IHC: human adrenal gland tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab118291 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCAM1 antibody (ab118291)Anti-NCAM1 antibody (ab118291) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to the glycosylated form of the protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody (ab118291)Immunohistochemical analysis of paraffin embedded human adrenal gland staining NCAM1 with ab118291 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.
