Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2567Y] to NCAM1 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free
    See all NCAM1 primary antibodies

  • Description

    Rabbit monoclonal [EP2567Y] to NCAM1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.

  • General notes

    ab215981 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    ab75813 at 1:1000 staining NCAM1 antibody in human astrocytoma tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling NCAM1 with ab75813 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Positive staining on tumor cells of human astrocytoma is observed. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75813).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981) This image is courtesy of an anonymous Abreview.

    ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75813).

  • Flow Cytometry - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Flow Cytometry - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Overlay histogram showing SH-SY5Y cells stained with ab75813 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75813, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75813).

  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    ab75813 at 1/100 dilution staining NCAM in SH-SY5Y cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75813).

  • Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)
    Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

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