Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23457-15] to Syntaxin 1a - BSA and Azide free
  • Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free
    See all Syntaxin 1a primary antibodies

  • Description

    Rabbit monoclonal [EPR23457-15] to Syntaxin 1a - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate; SH-SY5Y, Neuro-2a and PC-12 whole cell lysates. IHC-P: Human, mouse and rat cerebrum and pancreas tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neurons and PC-12 cells. Flow Cyt: Neuro-2a cells. IP: Human brain tissue lysate.

  • General notes

    ab273156 is the carrier-free version of ab272736. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Syntaxin 1a was immunoprecipitated from 0.35 mg human brain tissue lysate 10µg with ab272736 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10µg.

    Lane 2: ab272736 IP in human brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272736 in human brain tissue lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Flow Cytometry - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Flow Cytometry - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Syntaxin 1a with ab272736 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Syntaxin 1a with ab272736 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing membranous staining in PC-12 cell line is observed. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on mouse liver (PMID: 10644452) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on rat liver (PMID: 10644452) is observed. 

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272736).

  • Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
    Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)

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