Anti-NCAM1 antibody [RNL-1] (ab9018)
![Anti-NCAM1 antibody [RNL-1] (ab9018)](https://www.abcam.com/ps/products/9/ab9018/Images/ab9018-339643-9018.jpg "Anti-NCAM1 antibody [RNL-1] (ab9018)")
Key features and details
- Mouse monoclonal [RNL-1] to NCAM1
- Suitable for: ICC, IHC-FoFr
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-NCAM1 antibody [RNL-1]
See all NCAM1 primary antibodies -
Description
Mouse monoclonal [RNL-1] to NCAM1 -
Host species
Mouse -
Specificity
Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180. -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-FoFr Rat
-
Immunogen
Tissue, cells or virus corresponding to Human NCAM1. This antibody was raised against the small cell lung cancer cell line NCI-H82.
Database link: P13591 -
Positive control
- IHC-P: Mouse brain tissue. IHC-Fr: Rat brain tissue. ICC/IF: Adult-derived human liver stem cells and hepatic stellate cells.
-
General notes
Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RNL-1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)
Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).
-
![Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)](https://www.abcam.com/ps/products/9/ab9018/Images/ab9018-274341-anti-ncam1-antibody-rnl-1-immunofluorescence.png)
Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018) Image cropped from Berardis et al., Public Library of Science One, 1, e86137, Fig. 3a.; DOI: 10.1371/journal.pone.0086137 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.
-
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (ab9018)](https://www.abcam.com/ps/products/9/ab9018/Images/ab9018-16000-anti-ncam1-antibody-rnl-1-immunohistochemistry.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (ab9018)ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
µ m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.NB: We recommend indirect amplification of immunofluorescence for ab9018
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
