Anti-NCAM1 antibody [EP2566(2)] (ab137086)
![Anti-NCAM1 antibody [EP2566(2)] (ab137086)](https://www.abcam.com/ps/products/137/ab137086/Images/ab137086-151665-anti-ncam1-antibody-ep25662-western-blot.jpg "Anti-NCAM1 antibody [EP2566(2)] (ab137086)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2566(2)] to NCAM1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NCAM1 antibody [EP2566(2)]
See all NCAM1 primary antibodies -
Description
Rabbit monoclonal [EP2566(2)] to NCAM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human NCAM1. The exact sequence is proprietary.
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Positive control
- Human cerebellum, oligodendroglioma and SH-SY5Y lysates; Human glioma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2566(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCAM1 antibody [EP2566(2)] (ab137086)All lanes : Anti-NCAM1 antibody [EP2566(2)] (ab137086) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human oligodendroglioma lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 94 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2566(2)] (ab137086)](https://www.abcam.com/ps/products/137/ab137086/Images/ab137086-151664-anti-ncam1-antibody-ep25662-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2566(2)] (ab137086)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM with ab137086 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NCAM1 antibody [EP2566(2)] (ab137086)](https://www.abcam.com/ps/products/137/ab137086/Images/ab137086-3-benefits-of-recombinant-antibodies.png)
Anti-NCAM1 antibody [EP2566(2)] (ab137086)
