Anti-Neuroserpin antibody [EPR23329-221] (ab269277)
![Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-5-anti-neuroserpin-antibody-epr23329221-western-blot-lysate.jpg "Anti-Neuroserpin antibody [EPR23329-221] (ab269277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23329-221] to Neuroserpin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Neuroserpin antibody [EPR23329-221]
See all Neuroserpin primary antibodies -
Description
Rabbit monoclonal [EPR23329-221] to Neuroserpin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human and mouse brain tissue lysate; Rat pancreas tissue lysate. IHC-P: Human and rat cerebrum, mouse pancreas tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23329-221 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)All lanes : Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution
Lane 1 : Human brain tissue lysate at 20 µg
Lane 2 : Human skeletal muscle tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human skeletal muscle (PMID: 9070919).
Exposure time: 3 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-1-anti-neuroserpin-antibody-epr23329221-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neuroserpin with ab269277 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID: 17040209). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-4-anti-neuroserpin-antibody-epr23329221-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Neuroserpin with ab269277 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: No staining on human liver (PMID: 9070919).
The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-6-anti-neuroserpin-antibody-epr23329221-western-blot-lysate1.jpg)
Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 114 secs.
-
![Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-7-anti-neuroserpin-antibody-epr23329221-western-blot-lysate2.jpg)
Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution + Rat pancreas tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 46 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-2-anti-neuroserpin-antibody-epr23329221-immunohistochemistry-mouse-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Neuroserpin with ab269277 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse pancreatic islet (PMID: 9407089). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-3-anti-neuroserpin-antibody-epr23329221-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (ab269277)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neuroserpin with ab269277 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum (PMID: 17040209). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Anti-Neuroserpin antibody [EPR23329-221] (ab269277)](https://www.abcam.com/ps/products/269/ab269277/Images/ab269277-8-benefits-of-recombinant-antibodies.png)
Anti-Neuroserpin antibody [EPR23329-221] (ab269277)