Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [1G4] to NCAM1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-NCAM1 antibody [1G4]
See all NCAM1 primary antibodies
Description

Mouse monoclonal [1G4] to NCAM1
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human NCAM1 aa 1-750. Produced in E. coli.
Database link: P13591

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Positive control
- WB: pCMV6-ENTRY NCAM1-transfected HEK-293 T whole cell lysate; HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 whole cell lysate. IHC-P: Human cerebellum and cortex tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

1G4
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for NCAM1 using ab230724 at 10 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724)

Formalin-fixed, paraffin-embedded human cortex tissue stained for NCAM1 using ab230724 at 10 μg/ml in immunohistochemical analysis.
Western blot - Anti-NCAM1 antibody [1G4] (ab230724)

All lanes : Anti-NCAM1 antibody [1G4] (ab230724) at 1/200 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY NCAM1-transfected HEK-293T whole cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.

Predicted band size: 95 kDa
Western blot - Anti-NCAM1 antibody [1G4] (ab230724)

All lanes : Anti-NCAM1 antibody [1G4] (ab230724) at 1/200 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2 whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 95 kDa

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