Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸ 10 µl 103 862 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MMP22 antibody [EPR7769] (ab181118)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7769] to MMP22
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-MMP22 antibody [EPR7769]
See all MMP22 primary antibodies
Description

Rabbit monoclonal [EPR7769] to MMP22
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain tissue lysate, Calu 3 cell lysate, SK OV 3 cell lysate, Human testis tissue, A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7769
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP22 antibody [EPR7769] (ab181118)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP22 with ab181118 at 1/250.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MMP22 antibody [EPR7769] (ab181118)

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP22 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.
Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)

All lanes : Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution

Lane 1 : Calu 3 cell lysate
Lane 2 : SK OV 3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 56 kDa
why is the actual band size different from the predicted?
Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)

Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Anti-MMP22 antibody [EPR7769] (ab181118)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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