Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MMP22 antibody [EPR7769] (ab181118)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7769] to MMP22
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MMP22 antibody [EPR7769]
    See all MMP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR7769] to MMP22

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain tissue lysate, Calu 3 cell lysate, SK OV 3 cell lysate, Human testis tissue, A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP22 antibody [EPR7769] (ab181118)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP22 antibody [EPR7769] (ab181118)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP22 with ab181118 at 1/250.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP22 antibody [EPR7769] (ab181118)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP22 antibody [EPR7769] (ab181118)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP22 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.

  • Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)
    Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)
    All lanes : Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution

    Lane 1 : Calu 3 cell lysate
    Lane 2 : SK OV 3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)
    Western blot - Anti-MMP22 antibody [EPR7769] (ab181118)
    Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 44 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?

  • Anti-MMP22 antibody [EPR7769] (ab181118)
    Anti-MMP22 antibody [EPR7769] (ab181118)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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