Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)
![Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)](https://www.abcam.com/ps/products/271/ab271840/Images/ab271840-2-ab51074274270antimmp14antibodyep1264yimmunohistochemistry.jpg "Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1264Y] to MMP14 - BSA and Azide free
- Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MMP14 antibody [EP1264Y] - BSA and Azide free
See all MMP14 primary antibodies -
Description
Rabbit monoclonal [EP1264Y] to MMP14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Human breast carcinoma, endometrium carcinoma and kidney tissues. Flow Cyt (intra): A549 cells. IP: A431 cell lysate.
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General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
ab271840 is the carrier-free version of ab51074. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1264Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counterstain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution.
PBS instead of the primary antibody was used as the negative control (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074). -
![Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)](https://www.abcam.com/ps/products/271/ab271840/Images/ab271840-4-ab51074314152antimmp14antibodyep1264yimmunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)ab51074 staining MMP14 in HT-1080 (human fibrosarcoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 1/1000 dilution (0.2 μg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as the counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and weakly membranous staining in HT-1080 cell line.
Negative control (bottom panels): MCF7 PMID: 19208838.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074).
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![Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)](https://www.abcam.com/ps/products/271/ab271840/Images/ab271840-3-ab51074274271antimmp14antibodyep1264yimmunoprecipitation.jpg)
Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)ab51074 (purified) at 1/20 dilution (2 µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.
Lane 1: A431 whole cell lysate 10ug
Lane 2: ab51074 + A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 whole cell lysateFor western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074). -
![Flow Cytometry - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)](https://www.abcam.com/ps/products/271/ab271840/Images/ab271840-5-ab51074314154antimmp14antibodyep1264yflowcytometry.jpg)
Flow Cytometry - Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)Flow cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HT-1080 (Human fibrosarcoma epithelial cell, Right) cells labeling MMP14 with ab51074 at 1/200 dilution (0.1 μg) (red). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) / black was used as the isotype control. Cells incubated with secondary antibody only (blue) was used as the unlabeled control. Gated on viable cells.
Positive control (Right panel): HT-1080 cells.
Negative control (Left panel): MCF7 cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074). -
![Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)](https://www.abcam.com/ps/products/271/ab271840/Images/ab271840-6-benefits-of-recombinant-antibodies.png)
Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)
