Anti-MMP3 antibody [EP1186Y] (ab52915)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1186Y] to MMP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-MMP3 antibody [EP1186Y]
See all MMP3 primary antibodies -
Description
Rabbit monoclonal [EP1186Y] to MMP3 -
Host species
Rabbit -
Specificity
79% identities with MMP10 -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanWB MouseRatHumanRecombinant fragment -
Immunogen
Synthetic peptide within Human MMP3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Raji, HEK293; mouse lung, placenta, brain, kidney liver lysate; rat brain, liver, spleen, kidney lysate; IHC-P: Rat kidney, mouse liver, human liver. ICC/IF: HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1186Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate
Lane 2 : HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate
Lane 3 : Mouse lung lysate
Lane 4 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 84 secondsBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1 & 2: 10 seconds
Lane 3: 3 minutes -
All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes
Lane 2 & 3: 4 seconds -
Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified) + Rat kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer: 5% NFDM/TBST
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Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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