Antibodies, Proteins, Kits and Reagents for Life Science

368 544 ₸ Product size

100 µl 368 544 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1186Y] to MMP3
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-MMP3 antibody [EP1186Y]
See all MMP3 primary antibodies
Description

Rabbit monoclonal [EP1186Y] to MMP3
Host species

Rabbit
Specificity

79% identities with MMP10

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human MMP3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
Positive control
- WB: Raji, HEK293; mouse lung, placenta, brain, kidney liver lysate; rat brain, liver, spleen, kidney lysate; IHC-P: Rat kidney, mouse liver, human liver. ICC/IF: HT-29 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1186Y
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)

All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate
Lane 2 : HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate
Lane 3 : Mouse lung lysate
Lane 4 : Mouse placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 84 seconds

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)

Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)

Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)

All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1 & 2: 10 seconds
Lane 3: 3 minutes
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)

All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes
Lane 2 & 3: 4 seconds
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)

Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified) + Rat kidney lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)

Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)

Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Anti-MMP3 antibody [EP1186Y] (ab52915)

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