Anti-MMP3 antibody (ab53015)
Key features and details
- Rabbit polyclonal to MMP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP3 antibody
See all MMP3 primary antibodies -
Description
Rabbit polyclonal to MMP3 -
Host species
Rabbit -
Specificity
MMP3 antibody detects endogenous levels of total MMP3 protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide derived from human MMP3 Peptide available as ab134501.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MMP3 antibody (ab53015)All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution
Lane 1 : Extracts from 293 cells, untreated.
Lane 2 : Extracts from 293 cells, treated with the immunising peptide.
Predicted band size: 54 kDa
Observed band size: 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (ab53015) This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma labeling MMP3 with ab53015 at 1/100 dilution. Tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using citrate buffer (pH 6.0). The tissue was blocked with 1% serum for 1 hour at 25°C. A biotin conjugated polyclonal goat anti-rabbit secondary antibody was used at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (ab53015)ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (ab53015)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (ab53015) This image is courtesy of an Abreview submitted by Torsten Lowin.Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
