Mouse MMP9 ELISA Kit (ab253227)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.91 pg/ml
- Range: 140.63 pg/ml - 9000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MMP9 ELISA Kit
See all MMP9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.2% Inter-assay Sample n Mean SD CV% Serum 3 5.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
38.91 pg/ml -
Range
140.63 pg/ml - 9000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 92% - 117% Serum 94 90% - 100% Tissue Extracts 92 85% - 101% Hep Plasma 109 102% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse MMP9 ELISA Kit (ab253227) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP9 protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP9 with 38.91 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MMP9 (gelatinase B) is a member of the matrix metalloproteinases family of zinc and calcium dependent endopeptidases. MMP9 is a 95kDa protein produced by keratinocytes, monocytes, macrophages and PMN leukocytes. It is able to degrade all the components of the extracellular matrix including gelatin, collagen types IV and V, elastin and proteoglycan core protein. MMP9 is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP9 is present in most inflammatory responses. Mouse MMP9 shares 72.9% sequence homology with primate. 71.8% with human, 81.6 % with rat, and 73.0% with bovine MMP9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MMP9 Capture Antibody 1 x 600µl 10X Mouse MMP9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse MMP9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
Database links
- Entrez Gene: 17395 Mouse
- SwissProt: P41245 Mouse
- Unigene: 4406 Mouse
Images
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Other - Mouse MMP9 ELISA Kit (ab253227)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MMP9 standard curve in Sample Diluent NS.The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse MMP9 standard curve in 1X Cell Extraction Buffer PTR.The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MMP9 in mouse serum and plasma - heparin samples.The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 158.72 ng/mL in serum and 37.15 ng/mL in plasma (heparin).
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Interpolated concentrations of native MMP9 in mouse LPS unstimulated and stimulated P388D1 cell culture supernatant samples.The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated P388D1 supernatant 100% and LPS stimulated P388 D1 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 1.03 ng/mL in unstimulated P388D1 supernatant and 74.58 ng/mL in LPS stimulated P388D1.
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Interpolated concentrations of native MMP9 in mouse J774A.1 cell culture supernatant samples.The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 cell culture supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 1326.73 ng/mL in J774A.1 cell culture supernatant.
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Interpolated concentrations of native MMP9 in mouse lung extract samples based on a 50 µg/mL extract load and spleen extract samples based on a 25 µg/mL extract load.The concentrations of MMP9 were measured in duplicate and interpolated from the MMP9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 5.51 ng/mL in lung extract and 5.37 ng/mL in spleen extract.
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Sandwich ELISA - Mouse MMP9 ELISA Kit (ab253227)To learn more about the advantages of recombinant antibodies see here.
