Anti-MMP12 antibody [EP1261Y] (ab52897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1261Y] to MMP12
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-MMP12 antibody [EP1261Y]
See all MMP12 primary antibodies -
Description
Rabbit monoclonal [EP1261Y] to MMP12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB HumanRecombinant fragment -
Immunogen
Synthetic peptide within Human MMP12 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P39900 -
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1261Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/2000 dilution (purified)
Lane 1 : HT-1080 cell lysate
Lane 2 : WI-38 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
ab52897 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Based on Western blot, unpurified ab52897 recognizes both pro and active MMP. The propeptide is 54 kDa, and 45 kDa and 22 kDa are active forms. The primary was used at 1:100 to detect recombinant MMP12 protein, but can be diluted more to decrease the intensity of the bands.
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