Anti-FAM3C/ILEI antibody (ab72182)
Key features and details
- Rabbit polyclonal to FAM3C/ILEI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FAM3C/ILEI antibody
See all FAM3C/ILEI primary antibodies -
Description
Rabbit polyclonal to FAM3C/ILEI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Pufferfish, Opossum -
Immunogen
Synthetic peptide corresponding to Human FAM3C/ILEI.
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General notes
This product was previously labelled as FAM3C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles
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All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate with immunising peptide
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
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ab72182 at 5µg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
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All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/ml
Lane 1 : Human testis tissue lysate with 5% non-fat milk in TBST
Lane 2 : Mouse testis tissue lysate with 5% non-fat milk in TBST
Secondary
All lanes : anti-rabbit HRP
Predicted band size: 25 kDa
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ab72182 at 10µg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.