Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free (ab271971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19816] to MLKL (phospho Y222) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free
    See all MLKL primary antibodies

  • Description

    Rabbit monoclonal [EPR19816] to MLKL (phospho Y222) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate

  • General notes

    ab271971 is the carrier-free version of ab206336. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free (ab271971)
    Western blot - Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free (ab271971)
    All lanes : Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with MLKL-WT expression vector, whole cell lysate at 20 µg
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysat at 4 µg
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate at 20 µg
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate at 20 µg
    Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y222F mutant expression vector, whole cell lysate at 20 µg
    Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218/222F mutant expression vector, whole cell lysate at 8 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lysates were kindly provided by our collaborator Dr. Xinhua Feng, Zhejiang University.

    The molecular weight of NPM-ALK is around 80 kDa (lane 2) [PMID: 11090048].

    This antibody can potentially cross react with other kinases.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206336).

  • Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free (ab271971)
    Anti-MLKL (phospho Y222) antibody [EPR19816] - BSA and Azide free (ab271971)

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