Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mkl1/MRTFA antibody (ab115319)

Key features and details

  • Rabbit polyclonal to Mkl1/MRTFA
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Mkl1/MRTFA antibody
    See all Mkl1/MRTFA primary antibodies

  • Description

    Rabbit polyclonal to Mkl1/MRTFA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in HepG2 and HEK293 as well as the following tissue lysates: Mouse Lung; Human Placenta; Human Small Intestine. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MEF1

  • General notes

     This product was previously labelled as Mkl1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab115319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.

  • Tissue specificity

    Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.

  • Involvement in disease

    Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.

  • Sequence similarities

    Contains 2 RPEL repeats.
    Contains 1 SAP domain.

  • Domain

    The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
    The RPEL repeats mediate binding to globular actin.

  • Cellular localization

    Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AI852829 antibody
    • AMKL antibody
    • AW743281 antibody
    • AW821984 antibody
    • Basic SAP coiled-coil transcription activator antibody
    • Bsac antibody
    • KIAA1438 antibody
    • Mal antibody
    • Megakaryoblastic leukemia 1 protein antibody
    • Megakaryocytic acute leukemia protein antibody
    • MKL (megakaryoblastic leukemia)/myocardin like 1 antibody
    • MKL/myocardin like protein 1 antibody
    • MKL/myocardin-like protein 1 antibody
    • Mkl1 antibody
    • MKL1_HUMAN antibody
    • MRTF A antibody
    • Mrtf A antibody
    • MRTF-A antibody
    • MRTFA antibody
    • MYOCARDIN RELATED PROTEIN antibody
    • Myocardin related transcription factor A antibody
    • Myocardin-related transcription factor A antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mkl1/MRTFA antibody (ab115319)
    Immunocytochemistry/ Immunofluorescence - Anti-Mkl1/MRTFA antibody (ab115319)

    ICC/IF image of ab115319 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115319 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Mkl1/MRTFA antibody (ab115319)
    Western blot - Anti-Mkl1/MRTFA antibody (ab115319)
    All lanes : Anti-Mkl1/MRTFA antibody (ab115319) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 99 kDa
    Additional bands at: 22 kDa, 35 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes

References (2)

Publishing research using ab115319? Please let us know so that we can cite the reference in this datasheet.

ab115319 has been referenced in 2 publications.

  • Zhang R  et al. miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke. Brain Res 1714:18-26 (2019). PubMed: 30779911
  • Li N  et al. Opposite effects of HDAC5 and p300 on MRTF-A-related neuronal apoptosis during ischemia/reperfusion injury in rats. Cell Death Dis 8:e2624 (2017). PubMed: 28230854

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mkl1/MRTFA antibody (ab115319)
    Immunocytochemistry/ Immunofluorescence - Anti-Mkl1/MRTFA antibody (ab115319)

    ICC/IF image of ab115319 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115319 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Mkl1/MRTFA antibody (ab115319)
    Western blot - Anti-Mkl1/MRTFA antibody (ab115319)
    All lanes : Anti-Mkl1/MRTFA antibody (ab115319) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 99 kDa
    Additional bands at: 22 kDa, 35 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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