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Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MDH1 antibody [EPR13597(B)] - C-terminal
    See all MDH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P40925

  • Positive control

    • Jurkat, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    Western blot - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    All lanes : Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    Immunocytochemistry/ Immunofluorescence - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.

  • Immunoprecipitation - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    Immunoprecipitation - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)

    Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution

  • Flow Cytometry - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    Flow Cytometry - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).

  • Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
    Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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