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Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (ab255724)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 64 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MDH1 ELISA Kit (cytosolic malate dehydrogenase)
    See all MDH1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 1.2%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    64 pg/ml

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 92 83% - 101%
    Hep Plasma 98 94% - 103%
    EDTA Plasma 96 88% - 103%
    Cit plasma 92 85% - 101%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (ab255724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDH1 (cytosolic malate dehydrogenase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDH1 (cytosolic malate dehydrogenase) with 64 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    MDH1 is the cytosolic form of malate dehydrogenase, an enzyme that catalyzes the oxidation of malate to oxaloacetate while concomitantly reducing NAD+ to NADH. Its classical role is the malate:aspartate shuttle, where it facilitates the transfer of electrons produced during glycolysis into the mitochondria to produce ATP. Beyond its classical role, this enzyme may play a primary regulatory role in adipogenesis.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human MDH1 standard curve in 1X Cell Extraction Buffer PTR.
    Example of human MDH1 standard curve in 1X Cell Extraction Buffer PTR.

    The MDH1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native MDH1 in human serum and plasma samples.
    Interpolated concentrations of native MDH1 in human serum and plasma samples.

    The concentrations of MDH1 were measured in duplicates, interpolated from the MDH1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDH1 concentration was determined to be 15.35 ng/mL in serum, 21.69 ng/mL in plasma (citrate), 15.07 ng/mL in plasma (heparin) and 13.22 ng/mL in plasma (EDTA).

  • Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (ab255724)
    Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (ab255724)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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