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Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)

Key features and details

  • Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B
  • Suitable for: ICC/IF, IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9]
    See all Lactate Dehydrogenase B/LDH-B primary antibodies

  • Description

    Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B

  • Host species

    Mouse

  • Specificity

    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HeLa cells and cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Previously labelled as Lactate Dehydrogenase B.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
    Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)

    ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.

  • Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
    Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)

    ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).

  • Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
    Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)

    Isolation of Lactate Dehydrogenase B/LDH-B using ab112996 by immunoprecipitation.
    The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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