Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
Key features and details
- Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9]
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B -
Host species
Mouse -
Specificity
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
Tested applications
Suitable for: ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa cells and cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Previously labelled as Lactate Dehydrogenase B.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
10E6AA9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
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ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
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Isolation of Lactate Dehydrogenase B/LDH-B using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.