Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MCM3 antibody [EPR7080] - BSA and Azide free
    See all MCM3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248222 is the carrier-free version of ab128923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7080

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Western blot - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    All lanes : Anti-MCM3 antibody [EPR7080] (ab128923) at 1/1000 dilution

    Lane 1 : L6 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : RAW 264.7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : K562 cell lysate
    Lane 8 : HepG2 cell lysate
    Lane 9 : U937 cell lysate
    Lane 10 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 91 kDa



    This data was developed using ab128923, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

    This data was developed using ab128923, the same antibody clone in a different buffer formulation.

    ab128923 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

    This data was developed using ab128923, the same antibody clone in a different buffer formulation.

    ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

    This data was developed using ab128923, the same antibody clone in a different buffer formulation.

    ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human tonsil tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Lung carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Flow Cytometry - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    This data was developed using ab128923, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Cervical carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
    Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

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