Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)
![Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)](https://www.abcam.com/ps/products/249/ab249480/Images/ab249480-393667-anti-wdr4-antibody-epr11052-western-blot-human-knockout-lysate.jpg "Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11052] to WDR4 - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-WDR4 antibody [EPR11052] - BSA and Azide free
See all WDR4 primary antibodies -
Description
Rabbit monoclonal [EPR11052] to WDR4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human WDR4. The exact sequence is proprietary.
Database link: P57081 -
Positive control
- WB: HeLa and HepG2 cell lysates.
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General notes
Ab249480 is the carrier-free version of ab169526. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249480 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11052 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)All lanes : Anti-WDR4 antibody [EPR11052] (ab169526) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : WDR4 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab169526).
Lanes 1-3: Merged signal (red and green). Green - ab169526 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab169526 Anti-WDR4 antibody [EPR11052] was shown to specifically react with WDR4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265109 (knockout cell lysate ab258285) was used. Wild-type and WDR4 knockout samples were subjected to SDS-PAGE. ab169526 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)](https://www.abcam.com/ps/products/249/ab249480/Images/ab249480-2-benefits-of-recombinant-antibodies.png)
Anti-WDR4 antibody [EPR11052] - BSA and Azide free (ab249480)