Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)
![Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)](https://www.abcam.com/ps/products/256/ab256523/Images/ab256523-2-anti-eef1a2-antibody-epr-22651-46-western-blot1-human.jpg "Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22651-46] to EEF1A2 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free
See all EEF1A2 primary antibodies -
Description
Rabbit monoclonal [EPR22651-46] to EEF1A2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, HEK-293T, Neuro 2a whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
ab256523 is the carrier-free version of ab227824.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22651-46 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)All lanes : Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : EEF1A2 knockout HAP1 whole cell lysate
Lane 3 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaab227824 was shown to specifically react with EEF1A2 in wild-type HAP1 cells as signal was lost in EEF1A2 knockout cells. Wild-type and EEF1A2 knockout samples were subjected to SDS-PAGE. ab227824 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes a weakly unidentifiable band at around 75kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3.25 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227824).
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![Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)](https://www.abcam.com/ps/products/256/ab256523/Images/ab256523-1-anti-eef1a2-antibody-epr-22651-46-immunoprecipitation-293t-human.jpg)
Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)EEF1A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab227824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Ab227824 1/1000 dilution (0.54 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2: ab227824 IP in HEK-293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227824 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227824).
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![Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)](https://www.abcam.com/ps/products/256/ab256523/Images/ab256523-3-benefits-of-recombinant-antibodies.png)
Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)
