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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-EEF1A2 antibody (ab227275)

Anti-EEF1A2 antibody (ab227275)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EEF1A2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EEF1A2 antibody
    See all EEF1A2 primary antibodies
  • Description

    Rabbit polyclonal to EEF1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Positive control

    • WB: Mouse and rat brain tissue lysate; HEK-293T, A431, NCI-H1299, HeLa S3, HepG2, MOLT-4 and Raji whole cell lysate. IHC-P: Mouse and rat fore brain tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Images

  • Western blot - Anti-EEF1A2 antibody (ab227275)
    Western blot - Anti-EEF1A2 antibody (ab227275)
    All lanes : Anti-EEF1A2 antibody (ab227275) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 7 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (ab227275)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (ab227275)

    Paraffin-embedded rat fore brain stained for EEF1A2 with ab227275 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-EEF1A2 antibody (ab227275)
    Western blot - Anti-EEF1A2 antibody (ab227275)
    Anti-EEF1A2 antibody (ab227275) at 1/5000 dilution + Mouse brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (ab227275)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (ab227275)

    Paraffin-embedded mouse fore brain stained for EEF1A2 with ab227275 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-EEF1A2 antibody (ab227275)
    Western blot - Anti-EEF1A2 antibody (ab227275)
    Anti-EEF1A2 antibody (ab227275) at 1/1000 dilution + Rat brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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