Antibodies, Proteins, Kits and Reagents for Life Science

Anti-EEF1A2 antibody - C-terminal (ab194441)

Key features and details

Mouse polyclonal to EEF1A2 - C-terminal
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment
Isotype: IgG

Product name

Anti-EEF1A2 antibody - C-terminal
See all EEF1A2 primary antibodies
Description

Mouse polyclonal to EEF1A2 - C-terminal
Host species

Mouse
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment (GST-tag) corresponding to Human EEF1A2 aa 350 to the C-terminus.
Database link: Q05639

Run BLAST with Run BLAST with
Positive control
- EEF1A2 recombinant protein; Human thyroid (diffuse hyperplasia) tissue lysate; PC12, Hela S3 NE and NIH 3T3 cell lysates.

Application	Species
WB	Mouse Rat Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Whole antiserum
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-EEF1A2 antibody - C-terminal (ab194441)

Anti-EEF1A2 antibody - C-terminal (ab194441) at 1/2500 dilution + EEF1A2 recombinant protein (immunizing peptide) at 0.2 µg

Predicted band size: 50 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.
Western blot - Anti-EEF1A2 antibody - C-terminal (ab194441)

Anti-EEF1A2 antibody - C-terminal (ab194441) at 1/1000 dilution + Human thyroid (diffuse hyperplasia) tissue lysate at 50 µg

Predicted band size: 50 kDa
Western blot - Anti-EEF1A2 antibody - C-terminal (ab194441)

Anti-EEF1A2 antibody - C-terminal (ab194441) at 1/1000 dilution + PC12 cell lysate at 50 µg

Predicted band size: 50 kDa
Western blot - Anti-EEF1A2 antibody - C-terminal (ab194441)

Anti-EEF1A2 antibody - C-terminal (ab194441) at 1/1000 dilution + Hela S3 NE cell lysate at 50 µg

Predicted band size: 50 kDa
Western blot - Anti-EEF1A2 antibody - C-terminal (ab194441)

Anti-EEF1A2 antibody - C-terminal (ab194441) at 1/1000 dilution + NIH 3T3 cell lysate at 50 µg

Predicted band size: 50 kDa

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