Anti-Isoleucyl tRNA synthetase antibody (ab264356)
Key features and details
- Rabbit polyclonal to Isoleucyl tRNA synthetase
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Isoleucyl tRNA synthetase antibody
See all Isoleucyl tRNA synthetase primary antibodies -
Description
Rabbit polyclonal to Isoleucyl tRNA synthetase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human Isoleucyl tRNA synthetase aa 1212-1262. The exact sequence is proprietary. NP_002152.2
Database link: P41252 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Isoleucyl tRNA synthetase antibody (ab264356)All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab264356) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 145 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Isoleucyl tRNA synthetase antibody (ab264356)Isoleucyl tRNA synthetase was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab264356 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264356 at 1 µg/ml.
Lane 1: ab264356 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
Cells prepared using NETN lysis buffer.
