Anti-Isoleucyl tRNA synthetase antibody (ab229643)
Key features and details
- Rabbit polyclonal to Isoleucyl tRNA synthetase
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Isoleucyl tRNA synthetase antibody
See all Isoleucyl tRNA synthetase primary antibodies -
Description
Rabbit polyclonal to Isoleucyl tRNA synthetase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment within Human Isoleucyl tRNA synthetase (internal sequence). The exact sequence is proprietary.
Database link: P41252 -
Positive control
- IHC-P: Rat brain tissue. IP: HEK-293T whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Isoleucyl tRNA synthetase antibody (ab229643)All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab229643) at 1/4000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 145 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isoleucyl tRNA synthetase antibody (ab229643)Paraffin-embedded rat brain tissue stained for Isoleucyl tRNA synthetase using ab229643 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Isoleucyl tRNA synthetase antibody (ab229643)Isoleucyl tRNA synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 µg ab229643. Western blot was performed from the immunoprecipitate using ab229643. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HEK-293T whole cell lysate.
Lane 2: ab229643 IP in HEK-293T whole cell lysate.
