Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
![Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62486-anti-methionyl-trna-synthetase-antibody-marsd10b4-western-blot.jpg "Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)")
Key features and details
- Mouse monoclonal [MARSD10B4] to MetRS/MARS
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-MetRS/MARS antibody [MARSD10B4]
See all MetRS/MARS primary antibodies -
Description
Mouse monoclonal [MARSD10B4] to MetRS/MARS -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human MetRS/MARS aa 50-200 (C terminal).
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Positive control
- WB: NIH3T3 whole cell, HeLa whole cell and F2408 whole cell lysates. IP: RIPA lysate of HeLa cells. ICC: HeLa cell. Flow Cyt: Hela cells.
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General notes
This product was previously labelled as Methionyl tRNA synthetase, MARS.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Ab50793 was filtered through an 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
MARSD10B4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62485-anti-methionyl-trna-synthetase-antibody-marsd10b4-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.
Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 5 µg + RIPA lysate of HeLa cells at 200 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62484-anti-methionyl-trna-synthetase-antibody-marsd10b4-western-blot.jpg)
Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
-
![Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62483-anti-methionyl-trna-synthetase-antibody-marsd10b4-western-blot.jpg)
Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + F2408 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
-
![Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62482-anti-methionyl-trna-synthetase-antibody-marsd10b4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
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![Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)](https://www.abcam.com/ps/products/50/ab50793/Images/ab50793-62481-anti-methionyl-trna-synthetase-antibody-marsd10b4-flow-cytometry.jpg)
Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.
