Recombinant human htrA4 protein (ab134446)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
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Product name
Recombinant human htrA4 protein
See all htrA4 proteins and peptides -
Biological activity
Proteolytic activity of ab134446 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 10 µg/ml HtrA4 within 24 hours at 37°C. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQL WGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARY EAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSL QNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGD VIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQML SLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNIN GKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETINHHHHHH -
Predicted molecular weight
38 kDa including tags -
Amino acids
138 to 476 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.816% Imidazole
Constituents: 0.64% Tris HCl, 15% Glycerol, 0.704% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human htrA4 protein (ab134446)SDS-PAGE analysis of ab134446 (1µg). Due to minor N-terminal truncation of the protein it may appear as a double band.
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SDS-PAGE - Recombinant human htrA4 protein (ab134446)Hydrolysis of ß-Casein by ab134446. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without htrA4 (10 µg/ml). After indicated time intervals 15 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
Lane 1: BSA-only control, 0 hours
Lane 2: BSA-only control, 24 hours
Lane 3: 1µg ab134446, 0 hours
Lane 4: 1µg ab134446, 6 hours
Lane 5: 1µg ab134446, 24 hours
