Human Progranulin ELISA Kit (ab252364)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 72.68 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Progranulin ELISA Kit
See all Granulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 5 6.7% Inter-assay Sample n Mean SD CV% Plasma 3 6.5% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
72.68 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 92% - 98% Saliva 94 90% - 99% Milk 98 88% - 108% Urine 89 86% - 91% Serum 101 95% - 106% Hep Plasma 119 114% - 123% EDTA Plasma 107 99% - 114% Cit plasma 106 97% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Progranulin ELISA Kit (ab252364) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Progranulin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Progranulin with 72.68 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Progranulin Capture Antibody 1 x 600µl 10X Human Progranulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Progranulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acrogranin
- CLN11
- GEP
see all -
Database links
- Entrez Gene: 2896 Human
- Omim: 138945 Human
- SwissProt: P28799 Human
- Unigene: 514220 Human
Images
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Other - Human Progranulin ELISA Kit (ab252364)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Progranulin Standard Curve ComparisonStandard Curve comparison between Human Progranulin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Progranulin standard curve in Sample Diluent NS.The Progranulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Progranulin in human serum and plasma samples.The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 6.25%, plasma (heparin) 6.25%, and plasma (EDTA) 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 60.9 ng/mL in serum, 47.8 ng/mL in plasma (citrate), 52.2 ng/ml in plasma (heparin), and 49.8 ng/ml in plasma (EDTA).
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Interpolated concentrations of native Progranulin in human milk, saliva, and cell culture supernatant samples.The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1: 160, saliva 5%, and OVCAR-3 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 360.2 ng/ml in milk, 60.4 ng/ml in saliva, and 24.4 ng/ml in OVCAR-3 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 67.7 ng/mL with a range of 43.7 – 124.8 ng/mL.
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Sandwich ELISA - Human Progranulin ELISA Kit (ab252364)To learn more about the advantages of recombinant antibodies see here.
