Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19048] to MAT1A + MAT2A
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-MAT1A + MAT2A antibody [EPR19048]
    See all MAT1A + MAT2A primary antibodies

  • Description

    Rabbit monoclonal [EPR19048] to MAT1A + MAT2A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, U937, HEK-293T, C6 and NIH/3T3 whole cell lysates; HepG2 untreated and treated with LPS whole cell lysates; Human MAT1A and MAT2A recombinant proteins; Human fetal liver, fetal heart, fetal kidney and fetal spleen lysates; Mouse liver, kidney and spleen lysates; Rat liver, brain and spleen lysates. IP: Ramos whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19048

  • Isotype

    IgG

Images

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Human MAT1A recombinant protein
    Lane 2 : Human MAT2A recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44,75 kDa
    why is the actual band size different from the predicted?



     

    Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human MAT1A recombinant protein contains aa1-395 with a His-Tag®. This recombinant protein was made in-house.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Lane 1 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution
    Lanes 2-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Lanes 1-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution
    Lanes 4-6 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



     

    Exposure times: Lane 1-2: 5 seconds; Lane 3-6: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile  observed is consistent with what has been described in the literature (PMID: 18695670 and PMID: 22879628).

  • Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg (Input).

    Lane 2: ab192846 IP in Ramos whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192846 in Ramos whole cell lysate.

     

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)
    Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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