Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19048] to MAT1A + MAT2A
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-MAT1A + MAT2A antibody [EPR19048]
See all MAT1A + MAT2A primary antibodies
Description

Rabbit monoclonal [EPR19048] to MAT1A + MAT2A
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Ramos, U937, HEK-293T, C6 and NIH/3T3 whole cell lysates; HepG2 untreated and treated with LPS whole cell lysates; Human MAT1A and MAT2A recombinant proteins; Human fetal liver, fetal heart, fetal kidney and fetal spleen lysates; Mouse liver, kidney and spleen lysates; Rat liver, brain and spleen lysates. IP: Ramos whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19048
Isotype

IgG

Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

Lane 1 : Human MAT1A recombinant protein
Lane 2 : Human MAT2A recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44,75 kDa
why is the actual band size different from the predicted?

Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

Human MAT1A recombinant protein contains aa1-395 with a His-Tag®. This recombinant protein was made in-house.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Lane 1 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution
Lanes 2-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Lanes 1-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution
Lanes 4-6 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Human fetal heart lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat kidney lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure times: Lane 1-2: 5 seconds; Lane 3-6: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 18695670 and PMID: 22879628).
Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Ramos whole cell lysate 10 µg (Input).

Lane 2: ab192846 IP in Ramos whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192846 in Ramos whole cell lysate.

Exposure time: 1 second.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Images