Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)
![Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138865-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg "Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5233] to LY75/DEC-205
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-LY75/DEC-205 antibody [EPR5233]
See all LY75/DEC-205 primary antibodies -
Description
Rabbit monoclonal [EPR5233] to LY75/DEC-205 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human LY75/DEC-205 aa 1700-1800 (intracellular). The exact sequence is proprietary.
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Positive control
- Human tonsil tissue; Human thymus, lymph node, tonsil and Mouse thymus lysates.
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General notes
Previously labelled as LY75.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.54 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5233 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)
Paraffin-embedded, formalin-fixed staining of LY75/DEC-205 with ab124897 in Human Tonsil tissue by immunohistochemistochemical analysis at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138864-anti-ly75-dec-205-antibody-epr5233-western-blot.jpg)
Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)All lanes : Anti-LY75/DEC-205 antibody [EPR5233] (ab124897) at 1/1000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Human lymph node lysate
Lane 3 : Human tonsil lysate
Lane 4 : Mouse thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 198 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138863-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing negative staining in Normal human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138862-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138861-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing positive staining in Normal human thymus tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138860-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing positive staining in Normal human spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138859-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing negative staining in human Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138858-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing positive staining in Normal human colon lymphocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138857-anti-ly75-dec-205-antibody-epr5233-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)ab124897 showing negative staining in Normal human lung tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-138856-anti-ly75-dec-205-antibody-epr5233.jpg)
OI-RD Scanning - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897) -
![Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)](https://www.abcam.com/ps/products/124/ab124897/Images/ab124897-11-benefits-of-recombinant-antibodies.png)
Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)
